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foxd3xenopus nerve 

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Experiment details for foxd3

Harney AS et al. (2012) Assay

Targeted inactivation of Snail family EMT regulatory factors by a Co(III)-Ebox conjugate.

Gene Clone Species Stages Anatomy
foxd3.L laevis NF stage 28 cranial nerve , nerve

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  Figure 5. Temperature modulation of Co(III)-Ebox-mediated Snail inhibition reveals a requirement for Snail in melanocyte formation. Neurula stage embryos injected with Co(III)-Ebox or Co(III)-EboxMut in the archenteron space were maintained at 14 and (A) neural crest migration visualized by whole mount in situ hybridization for Twist expression. Krox20 is used to visualize rhombomeres 3/5 in the hindbrain. Untreated n = 8; Co(III)-EboxMut n = 8; and Co(III)-Ebox n = 12. (B) Quantification of mandibular, hyoid and branchial streams of neural crest cells are represented as the distance migrated as a percentage of control embryos. T-tests determined that neither Co(III)-EboxMut nor Co(III)-Ebox had a significant effect on neural crest cell migration. (C) Sibling embryos were maintained until swimming tadpole stages at 14 (top panel) or 27 (bottom panel) when melanocyte formation could be assessed. Arrowheads point to regions of diminished melanocytes. For embryos maintained at 14, untreated n = 12; Co(III)-EboxMut n = 12; and Co(III)-Ebox n = 12. For embryos maintained at 27, untreated n = 12; Co(III)-EboxMut n = 12; and Co(III)-Ebox n = 13. (D) Same embryos were maintained until swimming tadpole stages at 14 (top panel) or 27 (bottom panel) when craniofacial cartilage formation could be assessed. Cartliage was stained with Alcian blue and dissected to visualize cartilage formation. (E) Injected embryos were reared at 14 (top panel) or 27 (bottom panel) until stage 28 and glial cell formation was visualized by whole mount in situ hybridization for FoxD3 expression. For embryos maintained at 14, untreated n = 15; Co(III)-EboxMut n = 17; and Co(III)-Ebox n = 11. For embryos maintained at 27, untreated n = 15; Co(III)-EboxMut n = 15; and Co(III)-Ebox n = 22. (F) Injected embryos were reared at 14 (top panel) or 27 (bottom panel) until stage 28 and primary neuron formation was visualized by whole mount in situ hybridization for N-tubulin expression. For embryos maintained at 14, untreated n = 15; Co(III)-EboxMut n = 17; and Co(III)-Ebox n = 13. For embryos maintained at 27, untreated n = 20; Co(III)-EboxMut n = 21; and Co(III)-Ebox n = 21.