| |
Figure 4. Immunofluorescence analysis of D–V foregut patterning and trachea-esophagus separation in Xenopus. A–O: Confocal immunostaining of the Xenopus foregut showing transverse sections at three positions along the anterior–posterior axis at the indicated stages showing protein expression of Sox2 (blue), Nkx2.1 (red), and FoxF1 (green). P: Transverse section through the E9.5 mouse foregut showing similar expression of Sox2 (blue) Nkx2.1 (red) and FoxF1 (green). Q,R: Transverse sections of the Xenopus foregut at NF36 and NF42 showing P63 expression (white) in the dorsal foregut (Q) and esophagus (R). Abbreviations: dfg, dorsal foregut; eso, esophagus; fge, foregut epithelium; h, heart; lb, lung bud; lpm, lateral plate mesoderm; nc, notochord; s, somite; st, stomach; tr, trachea; ubb, ultimobrachial body; vfg, ventral foregut. Scale bar = 0.1 mm.Download figure to PowerPoint |
