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Figure 3. Whole embryo in situ hybridization for pax6 and Xbf1. A: A stage-15 embryo viewed frontally. The pax6-expression pattern is restricted to anterior lateral plate areas (including the lateral neural ridge) and a small bridge along the most anterior neural plate. pax6 expression is first detected at stage 12.5. The small arrow indicates the anterior extent of Xbf1 expression. B: A stage-13/14 embryo viewed ventrofrontally. The Xbf1-expression pattern (indicated by the small arrow) is within the anterior neural ridge tissue and a small portion of the anterior neural plate. The most ventral areas of Xbf1 expression overlap with the most anterior borders of pax6 expression, especially along the medial neural ridge. C: A stage-29/30 embryo. The Xbf1-expression pattern includes the most anterior prospective telencephalon, the branchial neural crest, and the dorsoanterior portion of the optic vesicle (arrow). Scale bars = 250 mu m in A,C. |
