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foxl1xenopus dorsal 

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Experiment details for foxl1

Mir A et al. (2008) Assay

Long- and short-range signals control the dynamic expression of an animal hemisphere-specific gene in Xenopus.

Gene Clone Species Stages Anatomy
foxl1 xenopus NF stage 9 to NF stage 10 dorsal

  Fig. 5. Notch signaling is responsible for the initial dorsal restriction and mosaic expression of FoxI1e. (A) Maternal Xotch mRNA is depleted to 20–25% with 15 ng of thioate-modified DNA oligo. The level of Xotch remains low in the blastula and gastrula. (B) FoxI1e expression is 2- to 3-fold upregulated by depletion of Xotch. In situ hybridization shows an expansion of FoxI1e expression (C). (D) Injection of the constitutively active Notch Intracellular Domain (NICD) causes downregulation of FoxI1e relative to controls, and injection of the dominant negative construct Su(H)-DBM causes an upregulation of FoxI1e (E). (F) NICD upregulates the Notch target ESR-1, and Su(H)-DBM downregulates it. (G) In situ hybridization for FoxI1e comparing control and Su(H)-DBM injected embryos at stage 9.5 indicates that more, and in the most severe cases, all of the sensorial-layer animal cap cells express FoxI1e. Dorsal is to the right in all embryos shown. Scale bars represent 200 μm.

Gene Clone Species Stages Anatomy
foxl1 xenopus NF stage 10 dorsal

  Fig. 6. Maternal Vg1 activates Notch signaling in the blastula to control FoxI1e expression. (A) Vg1-depleted embryos were injected with 50 or 500 pg of NICD mRNA at the 2-cell stage. NICD rescued the increase in FoxI1e expression caused by Vg1 depletion. (B) These results were confirmed by in situ hybridization for FoxI1e at stage 10, which shows an upregulation of FoxI1e in Vg1-depleted embryos, and a reversal of this upregulation by subsequent injection with NICD. The control embryo is oriented with dorsal to the right. Depletion of Vg1 results in a delay of gastrulation, and so the orientations of both the Vg1-depleted and the NICD-rescued embryos are indeterminate. Scale bars represent 200 μm. (C) Real-time PCR at stage 10 shows that the Notch target ESR-1 is downregulated in Vg1-depleted embryos relative to controls, indicating that Notch signaling depends on Vg1 at this stage. (D) 200 pg of Vg1 mRNA was unable to rescue the increase in FoxI1e expression induced by loss of Notch signaling by injection of 500 pg Su(H)-DBM mRNA.