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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
gabra2	xenopus		midbrain [+] anterior tectum,cell body layer of the optic tectum,neuropil of the optic tectum,posterior tectum,radial glial cell,ventricular layer of the optic tectum,optic tectum

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Experiment details for gabra2

Cloning and characterization of GABAA α subunits and GABAB subunits in Xenopus laevis during development.

Cloning and characterization of GABAA α subunits and GABAB subunits in Xenopus laevis during development.

Gene	Clone	Species	Stages	Anatomy
gabra2.L		laevis	NF stage 24 to NF stage 26	midbrain
gabra2.L		laevis	NF stage 29 and 30 to NF stage 31	midbrain
gabra2.L		laevis	NF stage 33 and 34 to NF stage 35 and 36	midbrain
gabra2.S		laevis	NF stage 33 and 34 to NF stage 35 and 36	midbrain

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Figure 2. Expression patterns of GABAA receptor α-subunits and GABAB receptors in developing X. laevis embryos using whole mount in situ hybridization. Embryos were viewed laterally with anterior to the left and dorsal to the top. The arrowheads indicate regions of expression. A, D, G, J, M, P, S: Stages 24–26, (tailbud stages). B, E, H, K, N, Q, T: Stage 29–31 (hatching stages). C, F, I, L, O, R, U: Stages 34–36 (early swimming tadpole stages). A–C: GABAAα1 expression. D–F: GABAAα2 expression. G–I: GABAAα3 expression. J–L: GABAAα5 expression. M–O: GABAAα6 expression. P-R: GABAB1 expression. S–U: GABAB2 expression. F′, H′, I′, P′, Q′, R′, S′, T′, U′: Magnified images of portions of embryos. fb, forebrain; r, retina; hb, hindbrain; mb, midbrain; pg, pineal gland; sc, spinal cord; V, cranial ganglion V; VII, cranial ganglion VII; IX, cranial ganglion IX. Scale bar = 1.0 mm; scale bar in inset = 0.25 mm.Download figure to PowerPoint

Gene	Clone	Species	Stages	Anatomy
gabra2.L		laevis	NF stage 24 to NF stage 26	midbrain

gabra2 [GABA A receptor, alpha 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (24-26), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885

Gene	Clone	Species	Stages	Anatomy
gabra2.L		laevis	NF stage 29 and 30 to NF stage 31	midbrain

gabra2 [GABA A receptor, alpha 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (29-31), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885

Gene	Clone	Species	Stages	Anatomy
gabra2.L		laevis	NF stage 33 and 34 to NF stage 35 and 36	midbrain

gabra2 [GABA A receptor, alpha 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (34-36), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885

Gene	Clone	Species	Stages	Anatomy
gabra2.S		laevis	NF stage 33 and 34 to NF stage 35 and 36	midbrain

Gene	Clone	Species	Stages	Anatomy
gabra2.L		laevis	NF stage 35 and 36	midbrain
gabra2.S		laevis	NF stage 35 and 36	midbrain

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Figure 3. Histological analysis of GABA receptor subunit expression in swimming tadpole (stage 35) X. laevis embryos. Transverse 18-μm sections along the anterior-posterior axis, with dorsal to the top. A–E: GABAAα1 expression. F–J: GABAAα2 expression. K–O: GABAAα3 expression. P–T: GABAAα5 expression. U–Y: GABAB1 expression. Z-DD: GABAB2 expression. Prime letters: Magnified portions of sections. W′: Paraffin section to show lack of otic vesicle staining. fb, forebrain; mb, midbrain; hb, hindbrain; asc, anterior spinal cord; psc, posterior spinal cord; r, retina; sc, spinal cord; V, cranial ganglion V; VII, cranial ganglion VII,; IX, cranial ganglion IX: oe; olfactory epithelium; Pt, pituitary gland. Scale bars = 0.3 mm.Download figure to PowerPoint