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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
golm1
|
|
laevis
|
NF stage 11
|
mesoderm
,
ventral marginal zone
,
dorsal marginal zone
|
golm1
|
|
laevis
|
NF stage 17
|
mesoderm
,
circumblastoporal collar
,
archenteron
,
gastrocoel roof plate
,
axial mesoderm
|
golm1
|
|
laevis
|
NF stage 25
|
otic vesicle
,
cement gland
,
pronephric mesenchyme
,
proctodeum
,
pronephric kidney
|
golm1
|
|
laevis
|
NF stage 31
|
otic vesicle
,
cement gland
,
pronephric kidney
,
pronephric tubule
|
golm1
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
cement gland
,
pronephric kidney
,
pronephric tubule
|
golm1
|
|
laevis
|
NF stage 39
|
cement gland
,
head region
,
pronephric duct
,
mouth primordium
,
late proximal tubule
,
[+]
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Figure 4. The expression pattern of golph2 during early development. A.
RT-PCR analysis of the temporal expression pattern of golph2. UE, unfertilized egg. RT(-), negative control of reverse transcription. Ornithine decarboxylase (ODC) was used as the RNA loading control. B. Western blotting analysis of golph2 expression during Xenopus embryogenesis. Beta-actin served as a protein loading control. C. Whole-mount in situ hybridization was conducted using a digoxigenin-labeled antisense probe for golph2. C. Embryos bisected prior to in situ hybridization. Orientation: animal top, dorsal right (C, D); dorsal top, ventral bottom (E, F), stages as indicated. The expression of golph2 is detected in the dorsal marginal zone and the derivative mesoderm at the gastrula stage. G. Lateral view, anterior towards the left; golph2 expression in the pronephros is detected between stages 25 and 39. Expression of golph2 in the otic vesicle and cement gland is also shown (H). Arrow heads indicate the signal. |