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Fig. 6. Comparison of the expression of XHRT1, Xhairy1 and Xhairy2b in the midline of early Xenopus embryos. Embryos were analyzed by whole- mount in situ hybridization with probes for XHRT1 (A, C, E, G, I, K), Xhairy2b (B, D, F, H, J, L) and Xhairy1 (M, N). Stages of development are indicated on the left for each line of embryos. (A, B) Vegetal view with dorsal up, (C, I, J, M) dorsal view with anterior right, (G, H, K, L, N) transverse vibratome sections. Xhairy2b is activated earlier than XHRT1 in the developing floor plate and their expression overlaps extensively. Xhairy1 is weakly expressed in the neural midline. It is only detected starting from late neurula stage in the superficial layer of the prospective floor plate cells. |
