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Figure 5. P14 cells stimulate muscle formation in the froglet blastema. A,B: Hepatocyte growth factor (HGF) -releasing P14 cells labeled with red fluorescent dye PKH26 were implanted into the froglet blastema (A), and after 2 weeks, implanted P14 cells were detected in the froglet blastema (B). C-J: An anterior-posterior series of sections made from a specimen shown in B was stained with MF20 antibody. Arrows indicate that clusters of differentiated muscle cells existed in the manipulated spike, and higher magnifications of the regions indicated by the arrows are shown in D,F,H,J. These well-organized muscle fibers seem to consist of the bundled multinucleate fibers. K: Immunostaining with anti-Pax7 antibodies shows Pax7-expressing cells detectable at the MF20-positive region. Scale bars = 400 mu m. |
