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Fig. 2. Expression of XHNF1 beta;. (A) Developmental RT-PCR analysis of XHNF1 beta; expression. Xenopus embryonal stages are indicated in the upper part of the panel. Ornithine decarboxylase (ODC) was used
as an internal control. (B,C,D,G,H) XHNF1beta; expression as detected by whole-mount in situ hybridization at blastula stage 8.5 (B) and
stage 9 (C), gastrula stage 10.25 (D), neurula stage 14 (G) and
tailbud stage 29 (H); expression of Xbra (E) and chordin (F) at gastrula stage are shown for comparison. Arrowheads indicate the embryo marginal zone (B), or the dorsal blastopore lip (D); arrows in
G and H point towards XHNF1β expression in neuroectoderm and pronephric system, respectively. |
