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Fig. 3. Suppression of hnRNP K expression by antisense MO. Embryos were unilaterally injected with two separate non-overlapping hnRNP K MOs (MO1 in A-D; MO2 in E). (A1,A2) Stage 10 gastrula; persistent maternal hnRNP K expression. (B1,B2) Stage 15 neural plate stage; suppression of hnRNP K expression on the injected side (left of broken line). Upper arrowhead, neural fold; lower arrowhead, somitic mesoderm, on the uninjected side. (C1,C2) Stage 22 tailbud stage; hnRNP K expression in myotome, spinal cord and brain (arrowheads, left to right, respectively) was suppressed on the injected side (C2) when compared with the uninjected side (C1). Because the animals are bent, the optical section in C2 also contained some cells from the uninjected side of the embryo (rectangle). (D,E) Three-day tadpole (stages 39 and 40); the uninjected side exhibited normal staining and morphology, but the injected side exhibited only background immunostaining and some defects in somites. (A1-C2,E) Confocal optical sections; (D) fluorescence dissecting microscopic view of whole animal. hnRNP K immunostaining, red; co-injected FITC-dextran, green. |
