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Figure 7. pERK detected by Western blotting and IHC. (A) Western blots for pERK and tERK in sets of pooled embryos collected, as indicated, between stages 30 and 41. Note that the signal for pERK was clearly increased in heparanase 2 knockdown embryos versus CTL MO embryos between stages 30 and 36. This effect was no longer apparent at stage 40/41 (B- E) Transverse section of the neural tube of stage 32 uninjected embryo, with the dorsal surface uppermost, showing (B) all nuclei stained with DAPI (C) heparanase 2 immunoreactivity (D) pERK immunoreactivity (E) merged image. pERK+ nuclei (two are
35& 36 indicated by arrows) and heparanase 2+ cells (two are indicated by arrowheads) are detected in the lateral zones of the tube. Note that the cells with strong nuclear pERK immunostaining and those with strong heparanase 2 signals tend to be mutually exclusive. The image is representative of experiments on three embryos. |
