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Fig. 4. Expression of shh and ihh in early Xenopus lung development. (A–E) WISH detection of expression of shh. (A) WISH shows absence of shh expression in the lung bud at stage 39. (B) shh expression at anterior part of lungs at stage 41. Transverse section shows the expression of shh in the anterior (C) and middle (D) part of lung epithelium, but not in posterior (E) epithelium of the lung bud at stage 41. (F–I) WISH detection of expression of ihh. No expression of ihh was detected in the lung bud at stage 39 (F) and stage 41 (G). (H and I) Transverse section indicates lack of ihh expressed in the anterior or posterior part of lungs at stage 41. Red arrows and dotted red circles indicate lungs. Abbreviations: es, esophagus; nc, notochord. |
