| |
Fig. 3. Expression of JAK1 mRNA in
adult testis. The sections of the testis fixed
with 4% paraformaldehyde in phosphatebuffered
saline (PBS) were treated with
antisense (A) or sense (C) probe for JAK1
mRNA. After detection of the signals, the
sections were stained with hematoxylin
and eosin (A’, C’). A section adjacent to
the antisense probe-treated section was
immunostained with anti-Xtr antiserum to
detect spermatogenic cell (B). a, a’, and b
are enlarged images of the areas surrounded
by the rectangles in A, A’ and B,
respectively. PG, primary spermatogonium;
SG, secondary spermatogonia; PC,
primary spermatocytes; RT, round spermatids;
MS, mature sperm. Note that PGs
are distinguished by the presence (arrowheads
in A and a) or absence (double
arrowhead in A and a) of JAK1 mRNA.
Scale bars indicate 50 lm in A, A’, B, C
and C’; and 20 lm in a, a’, and b. |
