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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
kcnc4	xenopus		axon

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Experiment details for kcnc4

Pollock NS et al. (2002) Assay

Expression of voltage-dependent potassium channels in the developing visual system of Xenopus laevis.

Gene	Clone	Species	Stages	Anatomy
kcnc4		tropicalis	NF stage 32 to NF stage 40	axon

Fig. 5. Voltage-dependent potassium 3.4 (Kv3.4)–like subunits are expressed in the Xenopus retina. A: Membrane-associated labelling with an anti-Kv3.4 antibody of all retinal and lens (L) cells in a stage 32 Xenopus retina. Arrow points to retinal ganglion cell (RGC) axon labelling at the vitreal surface. B,C: A stage 33/34 retina at low (B) and high (C) power shows abundant labelling of RGC processes at the vitreal surface (vs). D: In a stage 37/38 retina, membrane-associated labelling of most retinal and lens cells is maintained, and staining in the synaptic layers appears (IPL and OPL, inner and out plexiform layers). E: Low magnification micrograph demonstrates Kv3.4 immunolabelling in the brain neuropil, the optic nerve, and retina at stage 40. CMZ, ciliary marginal zone; D, dorsal; Mb, midbrain; NR, neural retina; onh, optic nerve head; V, ventral. Scale bar 50 m for A, B, and D, 100 m for E, and 20 M for C.

Gene	Clone	Species	Stages	Anatomy
kcnc4		tropicalis	NF stage 33 and 34 to NF stage 40	axon

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Fig. 3. Voltage-dependent potassium (Kv) subunit-like immunoreactivity in developing retinal ganglion cells (RGCs) and their axons. A: Double labelling of a stage 33/34 retina with anti-Kv1.3 (green) and mouse anti-bromodeoxyuridine (BrdU; red) antibodies shows that in the central retina Kv1.3-like immunopositive cells (arrows) are not BrdU positive. B: Double labelling (yellow) with antibodies against the rat Kv4.2 subunit and islet-1 at stage 33/34. The islet-1 antibody stains all RGCs (red), but the Kv4.2 antibody (green) labels only a subset of these islet-1–positive RGCs. C: Double labelling (yellow) of a stage 41 retina with a Muller glial marker (3CB2) and the anti- Kv4.2 antibody shows that radial cell processes in the central third of the retina are immunopositive (arrows) for both markers, whereas processes in the peripheral retina are immunopositive for the Kv4.2- like subunit only (arrowheads). By this stage the optic nerve head (onh), but not RGC somata, are Kv4.2-like immunopositive. D: Merged image of E and F shows colocalization of the Kv3.4 subunit and actin filaments in an RGC growth cone. E: Actin labelled with rhodamine phalloidin. F: The Kv3.4 antibody labels the axon and growth cone. IPL, inner plexiform layer; L, lens; OPL, outer plexiform layer; RGCL, RGC layer. Scale bar 100 m for A–C and 10 m for D–F.