| |
Fig. 5. Voltage-dependent potassium 3.4 (Kv3.4)–like subunits are
expressed in the Xenopus retina. A: Membrane-associated labelling
with an anti-Kv3.4 antibody of all retinal and lens (L) cells in a stage
32 Xenopus retina. Arrow points to retinal ganglion cell (RGC) axon
labelling at the vitreal surface. B,C: A stage 33/34 retina at low (B)
and high (C) power shows abundant labelling of RGC processes at the
vitreal surface (vs). D: In a stage 37/38 retina, membrane-associated
labelling of most retinal and lens cells is maintained, and staining in
the synaptic layers appears (IPL and OPL, inner and out plexiform
layers). E: Low magnification micrograph demonstrates Kv3.4 immunolabelling
in the brain neuropil, the optic nerve, and retina at stage
40. CMZ, ciliary marginal zone; D, dorsal; Mb, midbrain; NR, neural
retina; onh, optic nerve head; V, ventral. Scale bar 50 m for A, B,
and D, 100 m for E, and 20 M for C. |
