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Fig. 6 DNRab11 alters asymmetric ion transporter localization at the 4-cell stage. (A) 4-cell embryos were oriented,
sectioned, and immunostained for ductin protein. Biases in expression were quantified by identifying the strongest
expressing blastomere(s). The control embryo shown (i) has the strongest expression in the ventral right cell, verified with
ImageJ thresholding tools (ii). The embryo injected with DNRab11 shown (iii) has the strongest expression in the dorsal left
cell, verified with ImageJ thresholding tools (iv). (B) A total of 110 control and 45 DNRab11-injected embryos were
quantitatively analyzed. In controls, the most common blastomere scored as having the highest expression of ductin was the
ventral right; ventral right localization was decreased in DNRab11 injected embryos, although this decrease did not reach
statistical significance. Dorsal left expression was rare in control embryos, and the incidence of dorsal left expression was
significantly higher in DNRab11-injected embryos (*p < 0.05). (C) The same protocol was used for identifying biases in the
expression of KCNQ1. The control embryo shown (i) has the strongest expression of KCNQ1 in the ventral right cell, verified
with ImageJ thresholding tools (ii). The embryo injected with DNRab11 shown (iii) has the strongest expression in the dorsal
left cell, verified with ImageJ thresholding tools (iv). (D) A total of 85 control and 40 DNRab11-injected embryos were
quantitatively analyzed. In controls, the most common blastomere scored as having the highest expression of KCNQ1 was
the ventral right; ventral right localization was decreased in DNRab11 injected embryos, although this decrease did not reach
statistical significance. Dorsal left expression was relatively rare in control embryos, and the incidence of dorsal left
expression was significantly higher in DNRab11-injected embryos (*p < 0.05). |