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letm1xenopus eye 

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Experiment details for letm1

Mills A et al. (2019) Assay

Wolf-Hirschhorn Syndrome-Associated Genes Are Enriched in Motile Neural Crest Cells and Affect Craniofacial Development in Xenopus laevis.

Gene Clone Species Stages Anatomy
letm1.L laevis NF stage 35 and 36 eye

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  FIGURE 2. WHS related genes are expressed in the migrating neural crest cells during embryonic development. (A,F) Lateral views of whole mount in situ hybridizations for twist, a CNC-enriched transcription factor. Arrows indicate the pharyngeal arches (PA). (B–E,G–J) In situ hybridizations for whsc1, whsc2, letm1, and tacc3 demonstrate enrichment in CNCs that occupy the PAs (n = 20 per probe, per timepoint). Scalebar is 250 μm.

Gene Clone Species Stages Anatomy
letm1.L laevis NF stage 35 and 36 eye

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  FIGURE S1 | Expression patterns for WHS related genes across early development. In situ hybridization utilized (A–F) antisense mRNA probe to whsc1, (G–L) full-length antisense mRNA probe to whsc2, (M–R) full-length antisense mRNA probe to letm1, and (S-X) 1 kb partial-length antisense mRNA probe to tacc3. Embryos shown at blastula stage (A,G,M,S), in dorsal view at stage 16–20 (B,H,N,T), in lateral view at stage 20–25 (C,I,O,U), detail of lateral anterior region at stage 35 (D,J,P,V), and in both lateral and dorsal views from stages 39–42 (E,K,Q,W and F,L,R,X). (Y) In situ hybridization probes generated against sense strands of WHS gene mRNAs, shown at stage 25. Brown coloration (S,T) is unbleached pigment, unrelated to in situ hybridization staining. Scalebar is 250 μm.bryos were anesthetized with benzocaine.