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Fig. 3. XLMO-2 expression pattern in embryos. RNA in situ hybridization analysis was performed with full-length antisense digoxigenin-labeled probes on albino Xenopus laevis embryos. (A) Stage 21; lateral, dorsal and ventral views of the same embryo showing extensive staining in the nascent ventral blood island (VBI), the tailbud region and the dorsal lateral plate (DLP) mesoderm (black arrowheads). (B) Stage 26; lateral view. Staining is evident in the VBI, DLP (black arrowhead) and tailbud region. Staining is also seen in the brain and in the eye. (C) Stage 33; lateral view. Expression of XLMO-2 is maintained at a high level in the VBI but has diminished in the DLP region (black arrowhead). Staining in the tailbud has condensed to a small region at the tip of the outgrowing tail. (D) Stage 37; lateral view. Staining is evident throughout the circulatory system as XLMO-2 expression is maintained in circulating primitive erythrocytes (asterisked arrowhead). Expression in the tailbud region has condensed to the very tip of the tail. (E) Transverse section of a stage 26 embryo (ventral half only). XLMO-2 stains the ventral blood island (hematopoietic) mesoderm. (F) Transverse section of a stage 21 embryo stained with XLMO-2. Staining is diffuse throughout the mesoderm of the nascent tail bud. |
