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Fig. 6. mab21l1 and myc are downstream of pax6. (A and B) In situ hybridization of wild-type (+/+) and pax6 mutant embryos (−/−, compound heterozygote of complex1 and 10-kbp inversion mutations, see Table S1 for sequence) at st, 21 (A) or st. 15 (B) with mab21l1 (A) or myc (B) probe. Red arrows indicate expression of mab21l1 (A) and myc (B) in the eye region. Expression of both genes is reduced in mutants. (C) In situ hybridization of wild-type (+/?) and rax mutant (−/−, see Fish et al., 2014) embryos with myc probe. Note that myc expression in the presumptive retina region (red arrow) is almost completely lost in the rax mutant as reported previously ( Fish et al., 2014), whereas in the pax6 mutant myc expression is consistently reduced, but to a lesser extent as shown in (B). White dotted circles show the presumptive lens ectoderm (PLE) region, in which expression of myc is indistinguishable between wild-type and rax mutant embryos. By contrast, expression of myc in the PLE of pax6 mutant is reduced compared to the wild-type embryo (B, white dotted circles). (D) In situ hybridization of wild-type embryos with pax6 (top left) and myc (top right) probes at stage 15. The same embryos are bisected at the level shown schematically, clearly showing the expression of pax6 and myc in the PLE (bottom, white dotted circles). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) |
