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Fig. 2. Embryos depleted of p27Xic1 protein fail to produce differentiated primary neurones. (A) Western blot for endogenous p27Xic1 protein levels on stage 18 embryos that were injected with 20 ng Con Mo or p27Xic1 Mo. Cytoskeletal-β-tubulin demonstrates equal loading. Con Mo injection has no effect on any of the neural markers examined (B, data not shown). (C-F) p27Xic1 Mo-injected embryos were analysed for expression of X-NGNR-1, XMyT-1, NeuroD and Nβtub (purple) by whole-mount in situ hybridisation, injected side towards the left (β-gal, light blue). Injection of 20 ng p27Xic1 Mo has no effect on X-NGNR-1 (C) but ablates XMyT-1 (D), NeuroD (E) and Nβtub (F) expression. |
