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Figure 5. A: Comparative in situ hybridization analysis of X. tropicalis Ngn1, Ngn2, Ngn3, and X. laevis Ngn1, Ngnr-1, and Ngn3 in stage 41/43 embryos. Probes are indicated on the left. Left panel shows a lateral view of the embryos, right panel shows a dorsal close up of the head region. Red arrowheads indicate the ventral Ngn3 expression in intestine B: Transverse section showing X. tropicalis Ngn2 expression in the ciliary marginal zone (CMZ) of the retina. C: Double in situ hybridization of analysis of X. laevis Ngn3 (dark blue) and Ptf1a/p48 (red) in stage 41 gut explants. Red arrowheads mark stained enteroendocrine cells. duo, duodenum; int, intestine; lt, lung tube; lv, liver; oes, esophagus; pa, pancreas, pr, proctodeum; st, stomach. |
