| |
Fig. 10. Cellular localization of the transmembrane receptor, p75 in frog epithelial tissues. Confocal and fluorescence light microscopic images show p75 (green) labeling in larval and mature frog corneas. (A) Representative region of a larval whole-mount cornea showing a few scattered p75 more brightly labeled cells in the peripheral cornea (pc), and a higher number of p75 brightly labeled cells distributed in the skin (sk) outside of the cornea. No p75 brightly labeled cells were observed in the central cornea (cc). Higher magnification confocal images of these regions are shown in B-G. (B–G) Distribution of p75 (green) labeled cells in the skin and the cornea of larval animals. (B′-G′) Merged images for B-G with Hoechst labeled nuclei (magenta). (B-B′) The apical epithelium of the skin has cytoplasmic localization of p75 in a higher number of cells (marked by white arrowheads). (C-C′) Moderate expression of p75 is detected in the peri-nuclear region of basal skin epithelial cells. (D-D′) p75 expression is undetectable in the apical epithelium of the central cornea. (E-E′) Basal cells of the central cornea have peri-nuclear expression of p75. (F-F′) High p75 expression is noted in a few apical epithelial cells of the peripheral cornea (marked by white arrowheads). (G-G′) Basal cells in the peripheral cornea have faint expression of p75 in the peri-nuclear region. (H–K) Immunofluorescent staining for p75 (green) on frozen sections of the adult frog epithelial tissues, as labeled. The apical surface is located towards the top of each image, and the basal surface towards the bottom. (H′-K′) Merged images for H-K with Hoechst labeled nuclei (magenta). (H-H′) p75 is expressed throughout the full thickness of the central corneal epithelium. The localization is membranous, as well as cytoplasmic. (I-I′) Epithelial cells present in all the layers of the limbal region have p75 staining. (J-J′) Ventral eyelid showing p75 localization. The epithelia of both inner and outer surfaces of the ventral eyelid have p75 expression. A few cells more brightly labeled with p75 are also detected (marked by white arrowheads). (K-K′) The skin epithelium has p75 expression in all the cellular layers. cc, central cornea; ive, inner ventral eyelid; ove, outer ventral eyelid; pc, peripheral cornea; sk, skin. Scale bar in K′ equals 60 μm in A, 25 μm for B-G, B′-G′, and 50 μm for H-K, H′-K’. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.) |
