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Figure 5 p75NTRa in situ hybridization in sections. I. CNS structures. (A) Transverse section
through stage 39 ventral telencephalon. Brackets indicate expression in ventral telencephalon in both
neuroepithelium and mantle zone. The neural tube is demarcated by a white dotted line. We did not
detect expression in olfactory epithelium above background. OE, olfactory epithelium. (B) Sense
strand control in same plane as (A). (C) Sagittal section through stage 45/46 retina showing
expression in the ganglion cell layer (innermost ring of cells, adjacent to lens), in the inner nuclear
layer (inner portion of outer ring of cells), and in the outer nuclear layer (outer portion of outer ring
of cells). No mRNA hybridization is observed in the inner plexiform layer, which contains the axons
of bipolar cells and dendrites of retinal ganglion cells. ipl, inner plexiform layer; le, lens; PE,
pigment epithelium. (D) Sense strand control. Anterior is to the left in (C) and (D). The epidermis
and pigment epithelium appear white in dark-field microscopy due to the presence of reflective
melanocytes, and not as a result of probe hybridization. Scale bar: 50 mm. |
