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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nr2f2
|
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laevis
|
NF stage 18
|
pre-chordal neural plate
|
nr2f2
|
|
laevis
|
NF stage 25
|
hindbrain
,
eye
,
hypothalamus
|
nr2f5
|
|
laevis
|
NF stage 18
|
pre-chordal neural plate
,
chordal neural plate
,
mesoderm
|
nr2f5
|
|
laevis
|
NF stage 28
|
hindbrain
,
mesoderm
,
cranial neural crest
,
intersomitic region
,
rhombomere
,
[+]
|
nr2f5
|
|
laevis
|
NF stage 31
|
diencephalon
,
mandibular crest
,
hyoid crest
,
cranial neural crest
,
pronephric kidney
,
[+]
|
nr2f5
|
|
laevis
|
NF stage 26
|
diencephalon
,
hindbrain
,
mandibular crest
,
hyoid crest
,
cranial neural crest
,
[+]
|
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Fig. 4. Whole mount in situ hybridization with an antisense xCOUP-TF-A RNA probe (A-D) and an antisense xCOUP-TF-B RNA probe (E-H). (A)
Lateral view of a stage 18 embryo; the arrow indicates the anterior border of the hindbrain expression domain. (B) Dorsal sideview of a stage 28 tailbud.
(C) Anterior part of a stage 31 embryo; an arrow indicates the mid-hindbrain border, rhombomeres are numbered. (D) Stage 26 embryo, stained
for a prolonged time to show the somite expression. (E) stage 18 embryo, arrow points to hindbrain expression. (F) stage 25 embryo, dorsal sideview.
(G) Anterior part of a stage 31 embryo, arrow indicates anterior border of hindbrain, rhombomeres are numbered. (H) Dorsal view of the head of a
stage 31 embryo. o in C, F, and G indicates the position of the otic vesicle. All embryos were cleared with Murray (benzyl alcohobbenzyl benzoate
1:2), except for B and F. Anterior is to the left. |
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Display additional annotations [+]
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Fig. 5. Transverse sections of embryos hybridized with an xCOUP-TF-A (A-C; stage 31) or an xCOUP-TF-B probe (D-l? stage 37). All sections are
slightly oblique, the dorsal side being more. posterior than the ventral side. (A,D) Section through the prosencephalic region. (B) Section at the eyelevel;
xCOUP-TF-A is expressed mainly in the branchial arches and ventrally in the eyes. (C) Section through the spinal cord. xCOUP-TF-A is expressed
in the ventral part of the spinal cord (arrows) and in the somites. (E) Section through the eyes and the hindbrain; neural staining is in rl . (F)
Section through the otic vesicle and r5. Abbreviations: ba, branchial arch; cg, cement gland; e, eye; nc, notochord; o, otic vesicle; p. prosencephalon; r,
rhombencephalon; s, somite; SC, spinal cord. Bars are 1OOpm (A,B) or 50pm (C-F). |