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Fig. 5. Localisation of xGCNF mRNA in germ cells. (A,B) Whole mount in situ hybridization of oocytes of stages I-V with xGCNF antisense (A) or
sense (B) RNA. Note that pigment is present in oocytes of advanced stages. Stages of oogenesis according to Dumont (1972) are indicated by Roman
numbers. Young and advanced stage IV are shown. (C,D) In situ hybridization on 20pm cryosections of ovary from a young frog (CD) was performed
with xGCNF antisense (C) or sense RNA (D). The maximum signal was obtained in previtellogenic (stage I) oocytes. Note the absence of
signal from somatic cells of the ovary. Bar, 250,~m. |
