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Fig. 6. Localization of xGCNF transcripts in embryos by whole mount in situ hybridization: Whole mount in situ hybridization of albino embryos with
Digoxigenin-labelled xGCNF antisense RNA reveals maximal expression of xGCNF transcripts during neurulation. Stages according to Nieuwkoop
and Faber refer to (A), early gastrula (stage lo), (B), late gastrula (stage 12), (C), early neurula (stage 14), (D), dorsal aspects of midneurula (stage 17)
and (E), dorsolateral aspect of late neurula (stage 21), (F), lateral aspect of early tailbud (stage 23). Anterior is to the left in (B-F). Note the anteroposterior
gradient of xGCNF mRNA expression at stages 12.5-21 (B-E) and labelling of the branchial arches (ba) at stage 23 (F). Bars, 0.5 mm. |
