| |
Fig. 3. In situ hybridization analysis of fullback RNA expression at gastrula and neurula stages. In situ hybridization was performed on intact (A,C,E,H) or bisected (B,D,F,G) albino Xenopus embryos. Dashed lines in panel (E) indicates the plane in which the embryo in (F) was cut in half. (A) Vegetal view of an early gastrula (stage 10). (B) Bisected embryo at early gastrula stage 10. (C) Posterior view of an embryo at mid-gastrula stage 11.5. (D) Embryo bisected sagittally at mid-gastrula stage 11.5. Note staining in involuted mesoderm. (E) Dorsal view of an embryo at early neurula stage 13. Arrows indicates stripes of staining on the dorsal side and dashed line indicates plane of section of embryo in (F). (F) Bisected embryo at early neurula stage 13. (G) Embryos bisected sagittally at early neurula stage 13. Note posterior staining. (H) Dorsal view of an embryo at late neurula stage 18. For all images, Vg, vegetal; D, dorsal; V, ventral; A, anterior; P, posterior. |
