Search Criteria |
Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
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nrp1 | xenopus | thalamus |
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Experiment details for nrp1
Takagi S et al. (1987) AssaySpecific cell surface labels in the visual centers of Xenopus laevis tadpole identified using monoclonal antibodies.
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FIG. 4. Whole mount of the diencephalon and mesencephalon of tadpoles (stage 51). (a) Retinal central pathways in a Xenopus tadpole. All retina1 axons from the right eye were labeled with HRP to show the projection to the visual centers, i.e., the optic tectum (OT), nucleus Belonci (NB), corpus geniculatum thalamicum (CGT), and basal optic nucleus (BON). OC: optic chiasm. For the method of HRP labeling, see Fujisawa et ul. (1981). (b) The tadpole brain stained with MAb-A5 by the ABC method. The optic tectum, the nucleus Belonci, and the corpus geniculatum thalamicum are positively stained. The staining of the basal optic nucleus is obscure. (c) The tadpole brain stained with MAb-B2 by the ABC method. All visual centers are negative (X40). | |||||||||||
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FIG. 2. Indirect immunofluorescence of MAb-A5 and MAb-B2 on cryostat sections of the diencephalon of Xenopus tadpoles (stage 53). (a) A coronal section stained with MAb-A5. The nucleus Belonci shows immunofluorescence (arrow) (X130). (b) The section next to (a) stained with MAb-B2. The nucleus Belonci is negative. (c) A coronal section of the diencephalic region that is located rostra1 to the region shown in (a). The corpus geniculatum thalamicum is stained with MAb-A5 (arrow). (d) The section next to (c) stained with MAb-B2. The corpus geniculatum thalamicum is negative. |