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Fig. 2. Pax7 antibody detection in stage 46 tadpole. Immunostaining with anti-Pax7 monoclonal antibody was carried out on transverse sections of stage 46 tadpoles. (A-C) Detection of Pax7 with DAB staining (brown). The tissues were counterstained with 0.5% Methyl Green solution. (A) Midbrain; (B) hindbrain; (C) spinal cord. (D) Immunostaining of Pax7 (red) and DAPI (blue) on cross-section of tadpole head. The arrow in D indicates expression of Pax7 in eye muscle. (E) Co-immunostaining of Pax7 (red) and laminin (green) on cross-section of tadpole tail muscle. Abbreviations: hb, hindbrain; mb, midbrain; pg, pituitary gland; sc, spinal cord. Scale bars: 20 μm. |
