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Fig. 2. Aggregation of pre existing β-cells give rise to bigger islets at the end of climax. (Aa–c) β-cell proliferation at different stages of metamorphosis. BrdU was injected intraperitoneally into staged animals every other day for 10 days and pancreases were collected on the 11th day from (a) NF55 tadpoles; (b) climax (NF59–NF66) and (c) growing froglets. BrdU (green) and insulin (red) were detected by antibodies. Cell cycle activity monitored by immunostaining with a PCNA antibody (green) and insulin (red). (d) NF55 tadpoles; (e) climax (NF59–NF66) and (f) growing froglets. (B) pElastase-GFP transgenic animal. (a–c) in situ hybridization of GFP mRNA; (d–f) immunohistochemistry for GFP (green) and insulin (red). (a, d) NF55; (b, e) NF62; (c, f) NF66. Scale bar 40 μm. |
