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Fig. 7. No correlation of &r-I expression with cell density or cell proliferation. (A) Localization of Oct-I protein in the stage 43 embryo as deter- mined by whole mount immunohistochemistry. (B) Spatial differences of cell density in the stage 44 embryo as determined by labeling with TOPRO- 3, a DNA binding dye. (C)Whole mount immunohistochemical staining of a stage 43 embryo performed with MAb PCIO, reactive with Proliferative Cell Nuclear Antigen (PCNA). High levels of PCNA are dt!ected dorso-laterally in the mid- and forebrain. |
