| |
Fig. 5. Cryostat sections showing the internal localisation of transcripts of X-CypA. Embryos at stages 26 (A), 33 (Bâ D) and 39 (E) were subjected to whole-mount in situ hybridisation, allowed to over-stain in developing solution, and then embedded in acrylamide. Blocks were frozen in isopentane and sectioned at 20â î¼m. Clear staining is observed in the sensory organs (eyes, otic vesicle) (panels Aâ C) and in the branchial arches (B). Some staining is observed in the central nervous system (A) and also in the peripheral nervous system (ganglion and spinal cord) (panels Câ E). The developing somites are also stained (panels D and E) as are the pronephric tubules (C) and duct (E). ba, branchial arches; c, cornea; df, dorsal fin; ed, epaxial dermomyotome; ev, eye vesicle; gl, ganglion; hd, hypaxial dermomyotome; l, lens; m, mesencephalon; n, neurons; no, notochord; ov, otic vesicle; pd, pronephric duct; pt, pronephric tubules; r, retina; rl, retinal layer; sc, spinal cord. |
