| |
Fig. 4. Spatial expression profiles of
polypyrimidine tract binding protein
(PTB) genes in late tailbud embryos.
Whole-mount in situ hybridization followed
by longitudinal and transverse sections
were performed to examine the spatial
expression of ptbp1, ptbp2 and ptbp3 in
late tailbud embryos. (A) ptbp1 (a) lateral
view of a stage 34 embryo, the white lines
indicate the level of transverse and longitudinal
sections shown in panels b-i, (b-d)
longitudinal sections of the head along the
dorso to ventral axis, (c longitudinal section
of the trunk realized at the same level as
panel c, (e-i) transverse sections along the
anterior to posterior axis, (i higher magnification
view of panel i. Black arrowheads
point to the CMZ of the retina (B) ptbp2
(a) lateral view of a stage 34 embryo, the
white lines indicate the level of transverse
and longitudinal sections shown in panels
b-g, (b-d) longitudinal sections of the head
at the level of the brain, (d longitudinal
section of the trunk realized in the same
plan as panel d, (e-g) transverse sections
of head and trunk. Black arrowheads point
to the neuronal retina. (C) ptbp3 (a) lateral
view of a stage 34 embryo, the white
lines indicate the level of transverse and
longitudinal sections shown in panels b-j,
(b-d) longitudinal sections of the head along
the dorso to ventral axis, (c longitudinal
section of the trunk realized at the same
level as panel c, (e,f) transverse sections
of the head at the level of the diencephal.
(g,h) higher magnification views of transverse
sections of the head at the level of
the rhombocephal, (i,j) transverse sections
of the trunk, (j higher magnification view
of panel j. Black arrowheads and arrows
point respectively to the posterior lens and
the hypothalamic labeling. Abbreviations
used are: da, dorsal aorta; di, diencephal;
dm, dermatome; h, heart; im, intermediate
mesoderm; m, myotome, nc, notochord, ov,
otic vesicle; rb, rhombocephal; sc, spinal
cord; va, ventral aorta. |
