| |
Fig. 2. FAK immunolabeling at the MTJ in dissociated Xenopus
tadpole myotomal muscle fibers. Tadpole tails were skinned, and
myotomal fibers were dissociated in collagenase. (A) Phase-contrast
image of the tip of one of these multinucleated fibers. Arrowhead
points to the position of a NMJ. Several satellite cells can be
observed in close association with the sarcolemma. (B) FITC-FAK
immunolabeling showing the distribution of FAK along the extent of
the MTJ at the fiber tip. MTJ infoldings are shown by the arrows.
(C) R-BTX-labeling of the NMJ (arrowhead). Comparison of the
FAK labeling with the NMJ (B-C) shows that the this protein is
absent from the postsynaptic AChR clusters. |
