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rab32xenopus eye 

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Experiment details for rab32

Voigt J et al. (2005) Assay

Expression cloning screening of a unique and full-length set of cDNA clones is an efficient method for identifying genes involved in Xenopus neurogenesis.

Gene Clone Species Stages Anatomy
rab32 tropicalis NF stage 26 eye

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  Fig. 5. Additional detail on the overexpression of 4 selected genes and Rab32 expression. (a) XER 81 RNA injections expand X-Sox3 (purple) on the injected side. (b+c) Frizzled10B RNA injections enhance the medial (motorneuron) stripe of N-tubulin expression (white arrowhead, in wholemount (B) and transverse (C) view), in addition to the ectopic expression of N-tubulin observed in the floorplate (shown in Fig. 4b). (dashed line-midline, l-lateral, i-intermediate, m-medial). (d+e) 1 μm sections of Rab32 (1 ng) injected (E) and control Xenopus laevis (D) embryos. (D) In wild type embryos the melanosomes (darkly pigmented vesicles, arrow) are localised predominantly under the apical surface, but some can be found deeper within the cells (arrowhead). In contrast, when Rab32 was overexpressed the melanosomes were clustered tightly together and localised under the apical surface (arrows). (f) Transverse section (30 μm) of a bleached Xenopus tropicalis tailbud embryo in situ hybridised with an antisense probe to Rab32. Rab 32 is expressed in the retinal pigment epithelium (purple).

Gene Clone Species Stages Anatomy
rab32 tropicalis NF stage 28 eye

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  Fig. 4. (a,b) Expression patterns of the single clones identified in the unique full-length set screen. Xenopus tropicalis gastrula, neurula and tailbud stage embryos were in situ hybridised with antisense probes to the single clones identified in the unique full-length set screen. Gastrula stages embryos are shown vegetally with dorsal to the top (unless stated otherwise). At neurula stages an anterior and dorsal view is presented. Tailbud embryos are oriented with anterior to the left and dorsal to the top unless indicated otherwise. In come cases the wholemount images are supplemented by neurula and tailbud stage dissected embryos. If no specific staining was detected no image is shown.

Gene Clone Species Stages Anatomy
rab32 tropicalis NF stage 28 eye

  rab32 (RAB32, member RAS oncogene family ) gene expression in Xenopus tropicalis embryos, NF stage 28, as assayed by in situ hybridization. Dorsal view: anterior left.