|
Display additional annotations [+]
|
|
Fig. 5. Additional detail on the overexpression of 4 selected genes and Rab32 expression. (a) XER 81 RNA injections expand X-Sox3 (purple) on the injected side. (b+c) Frizzled10B RNA injections enhance the medial (motorneuron) stripe of N-tubulin expression (white arrowhead, in wholemount (B) and transverse (C) view), in addition to the ectopic expression of N-tubulin observed in the floorplate (shown in Fig. 4b). (dashed line-midline, l-lateral, i-intermediate, m-medial). (d+e) 1 μm sections of Rab32 (1 ng) injected (E) and control Xenopus laevis (D) embryos. (D) In wild type embryos the melanosomes (darkly pigmented vesicles, arrow) are localised predominantly under the apical surface, but some can be found deeper within the cells (arrowhead). In contrast, when Rab32 was overexpressed the melanosomes were clustered tightly together and localised under the apical surface (arrows). (f) Transverse section (30 μm) of a bleached Xenopus tropicalis tailbud embryo in situ hybridised with an antisense probe to Rab32. Rab 32 is expressed in the retinal pigment epithelium (purple). |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gtf2f1
|
|
tropicalis
|
NF stage 17
|
neural plate
,
optic field
,
preplacodal ectoderm
,
anterior placodal area
|
gtf2f1
|
|
tropicalis
|
NF stage 26
|
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
branchial arch
,
eye
,
[+]
|
srsf5
|
|
tropicalis
|
NF stage 11
|
marginal zone
|
srsf5
|
|
tropicalis
|
NF stage 17
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
lateral plate mesoderm
,
cranial neural crest
,
[+]
|
srsf5
|
|
tropicalis
|
NF stage 28
|
retina
,
brain
,
midbrain
,
hindbrain
,
ventral blood island
,
[+]
|
zgpat
|
|
tropicalis
|
NF stage 11
|
dorsal marginal zone
|
zgpat
|
|
tropicalis
|
NF stage 17
|
neural crest
,
neural plate
,
cranial neural crest
|
zgpat
|
|
tropicalis
|
NF stage 28
|
brain
,
midbrain
,
hindbrain
,
head region
,
somite
,
[+]
|
prkacb
|
|
tropicalis
|
NF stage 11
|
marginal zone
|
prkacb
|
|
tropicalis
|
NF stage 17
|
neural plate
|
prkacb
|
|
tropicalis
|
NF stage 28
|
notochord
,
pharyngeal arch
,
eye
|
triqk
|
|
tropicalis
|
NF stage 24
to
NF stage 28
|
cement gland
,
cement gland primordium
|
dlx5
|
|
tropicalis
|
NF stage 11
|
animal hemisphere
,
animal pole
|
dlx5
|
|
tropicalis
|
NF stage 17
|
cement gland primordium
,
cranial neural crest
,
migratory neural crest cell
,
neural plate border
,
posterior placodal area
,
[+]
|
dlx5
|
|
tropicalis
|
NF stage 26
|
otic vesicle
,
forebrain
,
cement gland
,
cephalic nerve
,
cephalic nerve
|
stk17a
|
|
tropicalis
|
NF stage 11
|
dorso-lateral marginal zone
|
stk17a
|
|
tropicalis
|
NF stage 28
|
pronephric duct
,
eye
,
anterior cardinal vein
|
csnk1d
|
|
tropicalis
|
NF stage 11
|
ectoderm
,
animal hemisphere
|
csnk1d
|
|
tropicalis
|
NF stage 17
|
pre-chordal neural plate
,
neural groove
,
optic field
,
preplacodal ectoderm
,
anterior placodal area
,
[+]
|
csnk1d
|
|
tropicalis
|
NF stage 24
|
spinal cord
,
floor plate
|
|
|
Fig. 4. (a,b) Expression patterns of the single clones identified in the unique full-length set screen. Xenopus tropicalis gastrula, neurula and tailbud stage
embryos were in situ hybridised with antisense probes to the single clones identified in the unique full-length set screen. Gastrula stages embryos are shown
vegetally with dorsal to the top (unless stated otherwise). At neurula stages an anterior and dorsal view is presented. Tailbud embryos are oriented with anterior
to the left and dorsal to the top unless indicated otherwise. In come cases the wholemount images are supplemented by neurula and tailbud stage dissected
embryos. If no specific staining was detected no image is shown. |
|
|
|
rab32 (RAB32, member RAS oncogene family ) gene expression in Xenopus tropicalis embryos, NF stage 28, as assayed by in situ hybridization. Dorsal view: anterior left.
|