| |
Figure S6. Related to Fig. 6; The subcellular localization of Rapgef5 and Rap
proteins. (A1) Rapgef5 is localized to the nuclei in a section of a stage 10 Xenopus
embryo. (A2 - 7) Rapgef5 is detected specifically in the nuclei of sectioned paraffin
embedded stage 28 Xenopus embryos but is reduced in morphants (Compare A2 and
A3, magenta Rapgef5, blue DAPI). The lines on the schematic in A4 represent the
plane of section shown in A4 and A5. A6 and A7 are higher magnification of the white
boxes in A4 and A5, respectively. (B, C) Rapgef5 localizes specifically to the nuclei of
mouse MEFs and human RPE cells. (D) With the exception of Rap1A all rap proteins
localize both to the plasma membrane and nucleus as assayed by injection of
GFP/RFP tagged constructs or by immunofluorescence. |
