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Fig. 3. Whole mount in situ hybridization analysis of embryonic hermes expression in Xenopus . (A) and (F) are whole mount in situs on albino embryos, (E) is a whole mount in situ on a pigmented embryo. (A) Early tailbud embryo (stage 26/27) showing expression of hermes in the heart primordia (black arrow) and the pronephric region (white arrow). (B) Ventral view of embryo shown in (A), indicating the paired heart primordia. (C) Stage 28 embryo, showing increase in hermes expression in the heart and pronephros. (D) Stage 36 embryo showing hermes expression in the heart, pronephros, eye and epiphysis (at the top of head). (E) Stage 34 embryo, showing onset of hermes expression in the eye. Note the punctate staining concentrated in the dorsal region of the eye. (F) Dorsal view of a stage 36 embryo showing paired epiphyseal staining on the top of the head. (G) Lateral view of a stage 36 embryo showing duct and tubule staining of the pronephros. (H) Section through the heart region of a stage 31 embryo shows hermes expression is limited to the myocardial layer of the heart and is absent from the endocardium. (I) Section through the anterior pronephros of a stage 35 embryo. hermes is expressed throughout the epithelium of tubules and glomus of the pronephros. (J) Section through the eye of a stage 36 embryo shows hermes expression in the ganglion layer, the inner-most cellular layer of the retina. E, endocardium; M, myocardium; L, lens; P, pigmented epithelium; T, tubules; G, glomus; S, somite. |
