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Fig. 2. Expression of Xenopus RDH10. (A,D) RT-PCR analysis of whole embryos (A) and embryonic explants (D). Histone H4 was used as an RNA loading control. (B,C,E-Z) Whole-mount in situ hybridization with an antisense RNA probe for XRDH10 (B,C,E,F,I-K,N,Q-X), XRALDH2 (G,L,O,Y) and XCYP26A1 (H,M,P,Z). Embryos are shown in lateral (B,C,Q,R,Y,Z), vegetal (E,G,H), dorsal (I,J,L,M) and anterior (N-P) views. Specimens are hemi-sectioned (F) and transversally sectioned (K,S-X). The asterisk in R indicates the midbrain-hindbrain boundary. alp, anterior lateral plate; cc, cardiac crescent; dac, dorsal animal cap; dbl, dorsal blastopore lip; ea, ear; ey, eye; hp, head process; mb, midbrain; n, notochord; nc, neural crest; ol, olfactory system; pba, posterior branchial arch; pm, presomitic mesoderm; pn, pronephros; pr, proctodeum; sc, spinal cord; te, telencephalon; vbl, ventral blastopore lip. |
