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Fig. 3. Spatial expression of XrhoB during gastrula stages detected by whole-mount in situ hybridization. Whole-mount in situ hybridization with XrhoB (A, B, F and G) and Xbra (D and I) probes. (A) Stage 10.25. Vegetal view. Dorsal side is on the right. XrhoB expression is localized at the dorsal marginal zone. (B) Stage 11. Vegetal view. Dorsal side is up. (C) Sagittal hemisection across the transverse C line of the embryo shown in (B). Dorsal side is on the right. Expression of XrhoB is detected in the involuting dorsal mesodermal cells (idm). (D) Xbra expression at stage 11. Vegetal view. (E) Sagittal hemisection across the transverse E line of the embryo shown in (D). Dorsal side is on the right. (F–G) Whole-mount in situ hybridization analysis of XrhoB at stage 12.5 (F) and 13 (G). Vegetal views. (H) Transverse paraffin section across the H line of the embryo shown in (G). Black arrowhead shows RhoB staining in posterior paraxial mesoderm. (I) Xbra expression at stage 13. Vegetal view. Abbreviations: bl, blastopore lip; idm, involuting dorsal mesoderm. |
