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Figure 4. IN-l immunocytochemistry on sections of the Xenopus CNS
and PNS. a, Schematic drawing of the Xenopus brain to indicate the
origin of sections b-e (arrows). band c, Cross sections through the spinal
cord (right arrow in a), exposed to antibodies against MBP (b) and IN-1
(c). White matter and myelinated fiber tracts crossing through the gray
matter are strongly positive for MBP and IN- 1. d and e, Section of the
optic nerve (left arrow in a). d, Immunostained with PLP antibodies to
reveal myelination. e, Mab IN-I does not bind to optic nerve myelin.
The immunostaining procedure produces unspecific staining of large
blood vessels (arrowhead in d), the meninges and epithelia around the
brain and PNS nerves (as in bi). fand g, Section of the optic tectum,
(middle arrow in a), immunostained with PLP antibodies (f), or immunostained
with Mab IN-l (g). No IN-I immunoreactivity is seen,
but myelinated tracts are present (f). h and i, Sections of the sciatic
nerve (PNS), in which the myelinated axons are stained by MBP-antibodies
(h) but not by IN-1 (i). All corresponding figures show consecutive
sections. Scale bars, 500 pm. |
