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Figure S1. Expression of Setd7 in the endoderm and developing pancreas is conserved
across vertebrate species. (A) Expression level of setd7 in Xenopus embryos at indicated
stages. A progressive increase in setd7 transcript levels is measured at neurula and tadpole
stages. Data were normalized to that of odc and represented as fold changes compared with
gastrula stage sample (set to 1). Error bars represent ± SEM. *P < 0.05. (B) Expression level of
Setd7 in the mouse foregut endoderm (E8.5) and pancreatic rudiments at indicated stages. Data
were normalized to that of Sdha and represented as fold changes compared with E8.5 (set to 1).
(C, D) Whole mount in situ hybridization (ISH) for setd7 in gastrula (st. 10+) and neurula stage (st.
14) Xenopus embryos. In C, yellow arrowheads indicate setd7 transcript in the dorsal cells
adjacent to the lip. In D, hemisected st. 14 embryo shows expression of setd7 in the anterior
ventral endoderm and middle dorsal portion of the archenteron roof (see yellow open
arrowheads). Both regions of the endoderm are fated to give rise to pancreas. (E) Whole mount
ISH analysis for foxa2 in hemisected neurula stage Xenopus embryos. foxa2 expression partially
overlaps with setd7 in the endoderm. (F-H) Immunofluorescence analysis of Setd7, Pdx1, Lamp1
and GM130 in E12.5 mouse pancreas. Arrows indicate cytoplasmic Setd7 and GM130. Size bar,
50 μm. (G) Immunofluorescence analysis of Setd7, Pdx1, and Insulin in E17.5 mouse pancreas.
Dotted circles demarcate acinar clusters negative for Setd7. Size bar, 50 μm. Abbreviations, Ecadherin,
E-cad; Hoechst, Hoe; Insulin, Ins. |
