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Figure 1. The spatio-temporal expression of Shank1 in Xenopus development. A–D: Lateral views of Xenopus embryos. A: At stage 28, Shank1 is expressed in the neural tube (green arrowhead). Dotted lines indicate the level of sections shown in E–H. B: Shank1 transcripts are detected in the neural tube (green arrowhead) and the trigeminal ganglion (gV) at stage 32. Dotted lines indicate the level of sections shown in I–K. C: At stage 35, Shank1 can be visualized in the neural tube (green arrowhead), the trigeminal ganglion (gV), as well as the anteriordorsal lateral line ganglion (gAD). Dotted line indicates the level of section in L. D: Shank1 is expressed in the posterior neural tube (green arrowhead), fore- and midbrain (blue and yellow arrowhead). In addition, Shank1 is detected in the trigeminal ganglion (gV) as well as the anteriordorsal lateral line ganglion (gAD) to this time point of development. E–H: Transverse sections through an embryo at stage 28. E: Shank1 mRNA molecules are visualized in the trigeminal ganglion (gV) and very weakly in the ventral neural tube (green arrowhead). F: Shank1 is expressed in the ventral neural tube (green arrowhead) and the anteriordorsal lateral line ganglion (gAD). Dotted lines surround the neural tube (nt) and the otic vesicle (ov). G,H: Shank1 transcripts are detected in the posterior ventral neural tube (green arrowhead). I–K: Transverse sections through an embryo at stage 32. I: Shank1 is expressed in the anteriordorsal lateral line ganglion (gAD) and the ventral neural tube (green arrowhead). J: Shank1 transcripts can be visualized in the proximal pronephros (red arrow). K: Shank1 mRNA molecules are located in the distal pronephric tubule (red arrowhead). L: Transverse section at stage 35. Shank1 is expressed in the ventral portion of the midbrain. In addition, Shank1 transcripts are detected intensely in the ganglion cell layer (GCL) and more weakly in the inner nuclear layer of the retina. e, eye; gAD, anteriordorsal lateral line ganglion; GCL, ganglion cell layer; gV, trigeminal ganglion; INL, inner nuclear layer; nt, neural tube; ONL, outer nuclear layer; ov, otic vesicle.Download figure to PowerPoint |
