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Figure 3. ProSAP2/Shank3 expression during Xenopus embryonic development. A: Lateral view of an embryo at stage 27. ProSAP2/Shank3 is expressed in the proximal tubule (pt) anlage of the pronephros. B: At stage 32, ProSAP2/Shank3 is predominantly expressed in the cardiovascular system. C: Ventral view. ProSAP2/Shank3 transcripts can be visualized in the ventral aorta (va) and the endocardium (ec). D, E: Lateral views of Xenopus embryos at stage 34 and 38. ProSAP3/Shank3 mRNA is localized in the cardiovascular system as indicated. F: Ventral view of an embryo at stage 38. ProSAP2/Shank3 is expressed in the ventral aorta (va) and the vitelline veins (vv). G–O: Transverse sections through Xenopus embryos at different stages as indicated. G: ProSAP2/Shank3 mRNA molecules are stained in the ventral interneurons of the neural tube (green arrowhead) as well as the developing head arteries (ha). H: ProSAP2/Shank3 is expressed in the endocardium (ec) of the heart. I: At stage 32, ProSAP2/Shank3transcripts can be visualized in the vitelline veins (vv). J: ProSAP3/Shank3 is expressed in the ventral interneurons of the neural tube (green arrowhead) and in the proximal tubule (pt) of the pronephros. K: At stage 32, ProSAP2/Shank3 transcripts are detected in the posterior cardinal veins (pcv) and in the media-lateral portion of the neural tube, the marginal and subventricular zones (green arrowhead). L: ProSAP2/Shank3 is expressed in the isthmus (yellow arrowhead) and the ventral-lateral neural tube. M: ProSAP3/Shank3 transcripts are detectable in the ventral interneurons of the midbrain (green arrowhead), the retina of the eye (white arrowhead), and the ventral aorta (va). N,O: ProSAP2/Shank3 is expressed in the medio-lateral neural tube (green arrowhead), the proximal tubule (N; pt) and connecting tubule (O; ct) of the pronephros, the vascular vitelline network (black arrowheads; vvn), as well as the hypochord (red arrowhead). The notochord is surrounded by a dotted line. aa, aortic arch arteries; acv, anterior cardinal vein; cg, cement gland; ct, collecting tubule of the pronephros; dc, Duct of Cuvier; e, eye; ec, endocardium; ha, head arteries; ia, intersomitic arteries; pcv, posterior cardinal vein; pt, proximal tubule of the pronephros; va, ventral aorta; vv, vitelline veins; vvn, vascular vitelline veins.Download figure to PowerPoint |
