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Fig. 1. Sulf1 is co-expressed with Shh in the floor plate of the neural tube in Xenopus tropicalis and is required for normal Shh signalling. in situ hybridisation shows the normal expression pattern of Sulf1 (A,C,E, G, I, K) and Shh (B, D, F, H, J, L) at stages 15 (A–D) 23 (E–H), and 35 (I–L). Arrows in (A, B, E, F, I, J) indicate level where the vibratome sections were taken at these same stages and are shown in (C, D, G, H, K, L). Sulf1 is expressed in the paraxial mesoderm at Stage 15 (A, C) while Shh is expressed in the floor plate and notochord (B, D). By stage 23, both Sulf1 and Shh are expressed in the floor plate (E–H), and this co-expression is still apparent at stage 35 (I–L). Knockdown of Sulf1 using a splice blocking morpholino oligo shows a reduced level of ptc expression in the neural tube (N, P) as compared to those injected with an equal amount of a control morpholino (M, O). |
