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Figure 5. Shroom Activity Is Required for Xenopus Neurulation (A) Xshroom is expressed exclusively in the neural plate during neural tube closure and then is maintained in the nasal and otic placodes (arrowhead and arrows, respectively). Dorsal view; anterior at top. Stages shown are 13, 15, 17, 18, and 20.(B) Expression of Shrm754–1108 disrupts anterior neural tube closure as evidenced by morphology (left), pax-3 expression (middle), and epidermal keratin expression (right). Embryos are shown at tailbud stage in dorsal views with anterior at top; neighboring schematics depict representative cross-sections.(C) Pax-3 expression also reveals consistent, but less-penetrant, defects in spinal neural tube closure. Dorsal view; anterior at left.(D) Shrm754–1108-induced neural tube defects are dose-dependent (N > 70 for each category). |
