| |
FIG. 4. Northern blots show the expression of the four Xenopu.;; Klps
during oogenesis and early development. (A) Probed with XKlpl, then
stripped and repro bed with XKlp2. The 288 band from the original gel
photograph is also shown. (B) Probed with XK!p3, then stripped and
reprobed with EFla, a ubiquitous gene product which codes for a
translation initiation factor, and used to check for RNA loading. (C)
Probed with the PCR fragmen t corresponding to XK\p4; RNA loading
is shown using the photograph of the 288 RNA in the original gel.
Roman numbers indicate oocyte stages and arabic numbers indicate
embryonic stages. |
