| |
Figure 5. Expression of SVOP in Xenopus embryo sections. A-K: Embryos were paraffin embedded and sectioned after whole-mount in situ hybridization. A-C: At stage 20, SVOP staining (A) correlates with differentiating Rohon-Beard neurons, as marked by Xaml (B) and XHox11L2 (C). D-F: An additional ventral domain of SVOP staining becomes apparent at stage 22 (D) and 25 (E), which mirrors the pattern of XNeuroD expression (F). G-I: At stage 30, ventrolateral SVOP staining was observed in the neural tube at trunk level (G), in the brain at the level of the otic vesicle (ov; H), and in the ventral forebrain (I). I: There was also staining of the trigeminal ganglion (tg; arrow) but no staining in the neural retina. J,K: At stage 42, frontal sections at the level of the retina (J) and at trunk level (K) reveal staining throughout the neural tube, with exclusion from the ventricular zone. J: There was also staining of the inner layers of the neural retina. ldquo n rdquo marks the notochord. |
