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tgfbixenopus liver 

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Experiment details for tgfbi

The XenopusTGFBI is required for embryogenesis throughregulation of canonical Wnt signalling.

The Xenopus Tgfbi is required for embryogenesis through regulation of canonical Wnt signalling.

Gene Clone Species Stages Anatomy
tgfbi.L laevis NF stage 42 liver

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  Fig. 2. Expression pattern of XTgfbi. (A) RT-PCR shows, in stage 10 embryos, XTgfbi was strongly expressed in DMZ and weakly expressed in VMZ and animal cap. Gsc and Xwnt-8 are markers for DMZ and VMZ, respectively. (B) Whole mount in situ hybridization of XTgfbi. Dashed lines indicate the position of transversal sections as labelled. (B) Dorsal view (rostral to the left) at stage 14. XTgfbi was weakly expressed in the dorsal midline. (C) Dorsal view at stage 16 showing a decreasing XTgfbi expression gradient in the dorsal midline. (D, D') Lateral and dorsal views at stage 22: XTgfbi expression was seen in the dorsal tips of developing somites. (E, F) Lateral and dorsal views at stage 25: XTgfbi was expressed in dorsal somite tips (arrowheads), weakly in tissues surrounding the eye (arrow). (G) Lateral view at stage 28: XTgfbi was expressed in intersomitic boundaries, around the eye, migrating cranial neural crests and tailbud. (H) Lateral view of embryo head at stage 33: XTgfbi expression was prominent in CNS (arrowhead), eye (arrow) and pitpharyngeal pouches (asterisk). (I) Side view at stage 33 showing strong expression in the somites, tailbud and migrating hypaxial muscle anlagen (arrowheads). (J) Lateral view at stage-36: XTgfbi expression is prominent in the migrating hypaxial muscle (arrowheads), eye and tail fin. (K) Lateral view at stage 39: XTgfbi is detected in migrating hypaxial muscle and tail fin (arrows). (L) Lateral view at stage 42: XTgfbi is expressed in ventral side of the embryo and migrating hypaxial muscle (arrowheads). (M, N, O) In situ hybridization with sense XTgfbi probe shows no specific staining. (P, Q) Transversal sections from C: XTgfbi was strongly expressed in the floor plateand notochord. (R, S, T) Transversal sections from G: XTgfbi was expressed in neural crest cells and somites. (U, V) Parasagittal sections from G: XTgfbi expression is in the somites, strong in the dorsal tips (asterisk) and intersomitic boundaries (arrowheads). (W) Transversal sections from I: XTgfbi expression is in olfactory pits, periphery of prosencephalon, migrating neural crests, pharyngeal pouches as well as rhombencephalon, otic vesicles and notochord. (Z) Sagittal section from L showing XTgfbi expression in tissues surrounding various internal organs and abdominal epithelia. fp, floor plate; int, intestine; liv, liver; mes, mesenchyme; nc, notochord; ncc, neural crest cell; olf, olfactory pit; ov, otic vesicle; pha, pharygneal pouch; rho, rhombencephalon; som, somite; stm, stomach. Scale bar=100 m.

Gene Clone Species Stages Anatomy
tgfbi.L laevis NF stage 42 liver

  tgfbi ( transforming growth factor, beta-induced, 68kDa ) gene expression in Xenopus laevis embryos, NF stage 42, as assayed by in situ hybridization. Lateral view: anterior left, dorsal up.