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FIG. 7. Hormone receptor gene expression in tadpole (A–C) and explant (D– F) larynges. Sections were hybridized in situ to S35 riboprobes
from the androgen receptor (A, B, D, E) or a sense control (F) or to the a isoform of the TR (C). AR mRNA is expressed in larynx, but not
pharynx, as early as tadpole stage 48 (A) and expression is also present at stage 54, even in the presence of PTU (B). In laryngeal explants,
AR mRNA expression is sustained after 16 days, with no apparent difference between untreated control explants (D) and TH/DHT-treated
explants (E). At stage 52, TRa mRNA is expressed in both larynx and pharynx (C). |