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Fig. 4. Immunohistochemical analysis of larval tissues for the p51/p63 proteins. (A) Localization of p51/p63-expressing cell in basal layer of epidermis. Xenopus, mouse, and zebrafish skin sections were stained with 4A4 and Nuclear Fast Red (Biomedia). Xenopus skin sections at indicated stages (st) and mouse sections on embryonic days (E) and that of newborn (NB) are shown. The frog sample at stage 60 displays the junction between the larval skin and the adult-type skin. Adult zebrafish epidermis with scales is shown. Bars indicate 50 μm. (B) Localization of the p51/p63 proteins in the frog tissues at stage 48. Tissues and organs are indicated. ONL, INL, IPL, and GNL in the retina indicate outer nuclear layer, inner nuclear layer, inner plexiform layer, and ganglion cell layer, respectively. The forelimb bud, gill, and velar plate sections were stained with 4A4 only. |
